Functional analysis of genes: from the design to the living system (FDBS), 5 ECTS, total teaching hours 36 + 4 for literature theme presentations by MSc students + >10 for familiarization with experimental manipulations and calculations in the laboratory

Aims: Understanding of modern research strategies through paradigms of development of molecular tools, model study systems and integrated analysis of important reference proteins, and familiarization with computational and wet-lab experimental methods.

Sections: I, Gene delineation, manipulation and expression. II, Protein function and interactions. III, Comprehensively studied protein paradigms. IV, Familiarization with dry-lab and wet-lab experimental methods. [section IV takes place in the laboratory and runs in parallel with the theoretical lessons (sections I-III) throughout the semester]

Themes taught and (approximate) duration:

Section I: Genetic and genomic engineering (4h), Mutagenesis designs (2h), Genome editing by CRISPR-Cas9 technology (2h), Bioinformatic analysis of genes (3h).

Section II: Protein interactions (4h), Quantification of protein interactions (2h), Methods of structure-function analysis (4h), Holistic approaches to protein analysis (OMICS) (3h), Model organisms – examples (4h).

Section III: Intracellular proteins: kinases or p53 (2h), Membrane proteins: GPCRs or SLCs (2h).

Section IV: Databases and software for analysis of biological data (2h), Statistical analysis of experimental results (4h), Experimental manipulations and calculations in the laboratory (>10h, in research labs).

E-course: http://ecourse.uoi.gr/course/view.php?id=1815

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