The biology of stem cells and applications in regenerative medicine (BSCRM), 5 ECTS, total teaching hours 45 + 4 for literature theme presentations by MSc students
Aims: Deeper understanding of principles of current biology through the study of stem cells and understanding of one of the most modern therapeutic approaches in the field of health biotechnology.
Sections: I, Types and properties of stem cells. IIA, Directed stem cell differentiation in vitro. ΙΙB, Applications in regenerative medicine
Themes taught and (approximate) duration:
Section I: Fertilization and pre-implantation embryo development / totipotency (2h), Post-implantation development – organogenesis (2h), Blastocyst cells / pluripotency (1h), Isolation and culture of embryonic stem cells (1h), Assays for pluripotency (1h), Primary spermatocytes (1h), Self-renewal / cell cycle (1h), Methods for maintaining self-renewal / pluripotency in vitro (2h), The gene regulation network of embryonic stem cells (2h), The epigenetic landscape of embryonic stem cells (2h), The signaling pathways of embryonic stem cells (2h), The shift from pluripotency to multipotency (2h), Somatic cell reprogramming / induced pluripotent stem cells (2h), «Plasticity» / transdifferentiation (1h), Adult stem cells / Niche (2h), Cancer “stem cells” (2h).
Section IIA: Differentiation of embryonic stem cells to various directions/transient states (2h), Differentiation of adult stem cells / precursors (2h), Signaling pathways and tissue-specific differentiation (2h), In vitro differentiation and malignant transformation (1h).
Section IIB: Inherent self-renewal capacity of tissues (1h), Production of tissues ex vivo, organoids (2h), Scaffolds / tissue mechanics (2h), Autologous transplants (1h), Cell replacement therapy (1h), Cell therapy of malignancies (2h).